Dr. David Sinclair's Supplement Protocol: The Sirtuin Longevity Stack, Available at X-Optimum

David Sinclair, Ph.D., is a professor of genetics at Harvard Medical School and co-director of the Paul F. Glenn Center for Biology of Aging Research. His 2019 book Lifespan: Why We Age, and Why We Don't Have To framed aging as a loss of information in the epigenome, not just wear and tear. He has repeated the same core personal protocol in interviews, including The Joe Rogan Experience and his own Lifespan podcast: raise NAD+, activate sirtuins, and keep cells under a mild metabolic stress that looks like caloric restriction.

This article is a catalog cross-reference, not an endorsement. X-Optimum has no affiliation with Sinclair, MetroBiotech, Tally Health, or Harvard. The compounds below come from his public comments. Prescription drugs he has mentioned (metformin, rapamycin, low-dose aspirin) stay in the "talk to a physician" column. Everything else maps to live products on x-optimum.com.

Primary search intent: David Sinclair supplement stack / David Sinclair supplement protocol.

The biology he is actually targeting

Sinclair's public stack is a sirtuin-first system.

Sirtuins (SIRT1–SIRT7) are NAD+-dependent deacetylases. They help keep DNA repair, mitochondrial biogenesis, and circadian clocks online. Two things starve them: falling NAD+ with age, and a constant fed state that never turns on AMPK or autophagy.

That is why his disclosed routine clusters around three levers:

1. NAD+ repletion via nicotinamide mononucleotide (NMN)
2. SIRT1 activation via trans-resveratrol taken with fat
3. CR-mimetic signaling via time-restricted eating, plus (in his case) metformin

If you only copy the capsule count and skip the fasting window, you miss the point of the protocol.

1. NMN: the NAD+ precursor he takes in the morning

Sinclair has repeatedly said he takes about 1 gram of NMN daily, often in the morning with yogurt. NMN is a direct precursor that cells convert to NAD+ through the NMNAT pathway. NAD+ is the electron-shuttle every dehydrogenase reaction needs, and it is the obligate cosubstrate of sirtuins and PARP DNA-repair enzymes.

Human NAD+ declines with age. The practical question is not whether NAD+ matters. It is whether oral NMN raises tissue NAD+ enough to matter. Early human work shows oral NMN is generally well tolerated and can shift blood NAD+ metabolites. Large, long-horizon outcome trials are still thin. Sinclair is explicit that he is running an n=1 experiment on himself, not writing a label claim.

X-Optimum match:

• Optimized NMN, $19.99. Use this as the precursor layer if you want the same compound class Sinclair discusses.
• Optimized NAD+, $21.99. Direct NAD+ plus quercetin and resveratrol for people who want the finished coenzyme plus a flavonoid partner, not only the precursor.

Take NAD+ work in the morning if you follow his timing. NAD+ biology is circadian. Night dosing can fight sleep pressure.

2. Resveratrol: the SIRT1 allosteric partner

Sinclair's lab helped make resveratrol famous as a SIRT1 activator. He has said he takes about 1 gram of resveratrol daily with yogurt so the fat carries a poorly water-soluble polyphenol.

Resveratrol is a stilbene concentrated in grape skins and berries. The trans-isomer is the bioactive form. Mechanistically it sits in two places at once: it can allosterically help SIRT1, and it hits AMPK, which then raises NAD+ via NAMPT. That is why he stacks it with NMN instead of treating them as substitutes.

X-Optimum match:

• Optimized Resveratrol, $19.99. Standalone trans-resveratrol for the sirtuin half of the stack.
• Superfood Reds Formula, $19.99. Food-matrix polyphenols (berries, phytochemicals) if you want the dietary pattern around the capsule, not a replacement for a measured resveratrol dose.

Take resveratrol with a fat-containing meal. Dry capsules on an empty stomach waste most of the dose.

3. The combined longevity layer

Sinclair's public comments treat NMN + resveratrol as one unit: fuel plus activator. X-Optimum already groups those SKUs.

• Longevity Bundle, $49.99. The shortest path if you want NAD+/NMN and resveratrol in one cart instead of three separate checkouts.

This is the closest catalog analog to the "Sinclair morning stack" people search for. It is still not his branded product and it is not a prescription protocol.

4. Quercetin and related flavonoids

Secondary write-ups of Sinclair's regimen often list quercetin and fisetin as senolytic-leaning flavonoids. Sinclair has discussed senolytics as a research class more than he has published a personal fisetin dose on the same level as NMN. Quercetin does appear alongside his NAD+ products in several public summaries.

X-Optimum's Optimized NAD+ ($21.99) already pairs NAD+ with quercetin and resveratrol. That is a legitimate biological overlap: quercetin is a flavonol with AMPK and senescent-cell research behind it, and it is not a forced catalog stretch.

Do not treat over-the-counter quercetin as a clinical senolytic cycle. Those protocols use pulse dosing under medical supervision.

5. Methyl donors, D3, and K2 (the support layer)

Public lists compiled from interviews also include:

• TMG (trimethylglycine / betaine) 500-1,000 mg, theoretically to donate methyl groups after NMN consumes nicotinamide
• Vitamin D3 4,000-5,000 IU
• Vitamin K2 180-360 mcg
• Omega-3, taurine, CoQ10 (doses less consistently stated)

X-Optimum does not sell standalone TMG or fish oil. The honest overlap is a complete micronutrient base, not a 1:1 TMG clone.

• Cognitive Support Multivitamin, $17.99
• Optimum Male Multivitamin for Men, $17.99
• Optimum Woman Multivitamin, $17.99 (handle: optimum-woman-multivitamin)

Use a multi for D and K coverage. If you add high-dose NMN, ask a clinician whether you also need a methyl donor. That is a lab question, not a blog claim.

6. What he does *not* put in a bottle

Sinclair's interviews spend as much time on behavior as on capsules.

Time-restricted eating. He has described skipping breakfast and keeping a long overnight fast so AMPK and autophagy stay online. Supplements do not replace that signal.

Metformin (evening, ~800-1,000 mg). AMPK agonist and diabetes drug. Not a supplement. Do not source it from a wellness cart.

Low-dose aspirin and rapamycin. He has discussed both in research and personal-experiment contexts. Both are drugs with bleeding and immune-suppression risk.

X-Optimum will not map those to a SKU. If a protocol needs a prescription, it needs a physician.

Sleep still matters in this system. SIRT1 talks to CLOCK/BMAL1. A fried circadian clock wastes NAD+ work.

• Optimized Sleep Formula, $17.99
• Better Sleep Strips, $17.99

How the pieces sit together

Build Your Sirtuin Longevity Stack at X-Optimum

Sinclair's public protocol is simple if you ignore the hype: restore the cosubstrate sirtuins burn (NAD+), give SIRT1 a polyphenol partner (trans-resveratrol), and keep a fasting signal so the system has a reason to turn on. X-Optimum stocks the two supplement pillars of that story, NMN/NAD+ and resveratrol, plus a reds formula and a sleep layer for the circadian half.

Start with Optimized NMN and Optimized Resveratrol, or grab the Longevity Bundle if you want them in one order. Browse the full catalog at https://x-optimum.com/collections/all.

Copy the pathways. Do not copy a Harvard professor's prescription cabinet.

Disclaimer: X-Optimum Wellness has no affiliation with David Sinclair. This article references publicly available information from David Sinclair's interviews, podcast, blog, and/or published works for educational purposes. Always consult a qualified healthcare provider before beginning any new supplement protocol.